Device

Part:BBa_K2314127:Design

Designed by: Lei Zhu   Group: iGEM17_OUC-China   (2017-10-22)


RFP Coding Device（Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
    Illegal AgeI site found at 728
    Illegal AgeI site found at 840
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any modifications involving the 5'UTR need to be considered whether or not a special mRNA secondary structure will be produced. The preliminary analysis of the secondary structure can be carried out by structural prediction software.The RBS sequence is TTGGGATGG.


Source

The researcher claimed that the PUTRs were precisely amplified without superfluous sequences based on the E. coli K12 transcriptional regulatory database, RegulonDB39。In addition, the sequence comes from the kit plate。Reference:【Zhou, S., Ding, R., Chen, J., Du, G., Li, H. Z., & Zhou, J. (2017). Obtaining a panel of cascade promoter-5'-utr complexes in escherichia coli. Acs Synthetic Biology, 6(6).】

References